About this Environmental Science article

Validation of a new yeast-based reporter assay consisting of human estrogen receptors α/β and coactivator SRC-1: Application for detection of estrogenic activity in environmental samples by Wai-Ling Chu; Kazuhiro Shiizaki; Masanobu Kawanishi; Mami Kondo; Takashi Yagi is a Environmental Science article available to read on EtoBox.

## Abstract Endocrine disruptors are exogenous substances that act like hormones in the endocrine system and disrupt the physiologic function of endogenous hormones. In the present study, we established reporter yeast strains (__Saccharomyces cerevisiae__) expressing human estrogen receptors, ERα or ERβ. These strains contain a reporter plasmid carrying an estrogen responsive element (ERE) upstream of the β‐galactosidase gene, and a plasmid expressing a steroid receptor coactivator, SRC‐1e. Using these reporter strains, we demonstrated dose‐dependent estrogenic activities of different categories of ligands, a natural hormone, 17β‐estradiol (E2); a synthetic drug, diethylstilbestrol (DES); phytoestrogens, genistein, daizein and emodin; and an environmental endocrine disrupter, bisphenol A. EC~50~ values of E2 for ERα and ERβ are 5.31 × 10^−10^ and 5.85 × 10^−10^ M, respectively. We also demonstrated that these yeasts were applicable for measuring estrogenic activities of environmental water samples. Most downstream sites of a river showed similar activity in both ERα and ERβ assays. These yeast strains are useful and convenient for detecting and comparing the estrogenic ligand activ

Author

Wai-Ling Chu; Kazuhiro Shiizaki; Masanobu Kawanishi; Mami Kondo; Takashi Yagi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1520-4081)

Published

2009

Language

EN

Field

Environmental Science (Physical Sciences)