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CHO gene expression profiling in biopharmaceutical process analysis and design by Jochen Schaub; Christoph Clemens; Peter Schorn; Tobias Hildebrandt; Werner Rust; Detlev Mennerich; Hitto Kaufmann; Torsten W. Schulz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is CHO gene expression profiling in biopharmaceutical process analysis and design about?

## Abstract Increase in both productivity and product yields in biopharmaceutical process development with recombinant protein producing mammalian cells can be mainly attributed to the advancements in cell line development, media, and process optimization. Only recently, genome‐scale technologies enable a system‐level analysis to elucidate the complex biomolecular basis of protein production in mammalian cells promising an increased process understanding and the deduction of knowledge‐based approaches for further process optimization. Here, the use of gene expression profiling for the analysis of a low titer (LT) and high titer (HT) fed batch process using the same IgG producing CHO cell line was investigated. We found that gene expression (i) significantly differed in HT versus LT process conditions due to differences in applied chemically defined, serum‐free media, (ii) changed over the time course of the fed batch processes, and that (iii) both metabolic pathways and 14 biological functions such as cellular growth or cell death were affected. Furthermore, detailed analysis of metabolism in a standard process format revealed the potential use of transcriptomics for rational media

Who reads CHO gene expression profiling in biopharmaceutical process analysis and design?

Author

Jochen Schaub; Christoph Clemens; Peter Schorn; Tobias Hildebrandt; Werner Rust; Detlev Mennerich; Hitto Kaufmann; Torsten W. Schulz

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)