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Achieving highly efficient rooting procedure in three Pyrus communis cultivars by response surface methodology by Atefe Ameri; Gholam Hossein Davarynejad; Nasrin Moshtaghi; Ali Tehranifar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Pyrus communis is a difficult-to-root species, and its optimal rooting depends on applying an accurate concentration of various chemicals. Since statistical modeling can be applied to estimate the approximate range of factors needed for the success of in vitro plant propagation, we tried to optimize the rooting medium using response surface methodology. Therefore, sucrose and polyvinyl alcohol were selected as independent variables in the central composite design of response surface methodology with α = 1. Based on the pre-test, it was found that the independent variables for the root formation were 2 to 3% sucrose and 0.5 to 1.5 g L -1 polyvinyl alcohol in studied cultivars. The statistical analysis results showed that the quadratic models were significant, with high R 2 values > 0.9 for rooting rate response. The optimized values for independent variables in rooting of the studied cultivars included 2% sucrose, 1.5 g L -1 polyvinyl alcohol in 'Williams'; 2.85% sucrose, 1.47 g L -1 polyvinyl alcohol in 'Natanzi'; and 3% sucrose, 0.99 g L -1 polyvinyl alcohol in 'Ghosi'. A separate validation experiment detected that the experimental values of the studied responses are in close agr

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Author

Atefe Ameri; Gholam Hossein Davarynejad; Nasrin Moshtaghi; Ali Tehranifar

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)