Can I read Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum on EtoBox?

Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum by A. Guri; K. C. Sink is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum about?

Mesophyll protoplasts of eggplant (cv Black Beauty) and of Solanum torvum (both 2n=2x=24) were fused using a modification of the Menczel and Wolfe PEG/DMSO procedure. Protoplasts post-fusion were plated at 1 × 10(5)/ml in modified KM medium, which inhibited division of S. torvum protoplasts. One week prior to shoot regeneration, ten individual calluses had a unique light-green background and were verified as cell hybrids by the presence of the dimer isozyme patterns for phosphoglucoisomerase (PGI) and glutamate oxaloacetate transaminase (GOT). Hybridity was also confirmed at the plant stage by DNA-DNA hybridization to a pea 45S ribosomal RNA gene probe. The ten somatic hybrid plants were established in the greenhouse and exhibited intermediate morphological characteristics such as leaf size and shape, flower size, shape, color and plant stature. Their chromosome number ranged from 46-48 (expected 2n=4x=48) and pollen viability was 5%-70%. In vitro shoots taken from the ten hybrid plants exhibited resistance to a verticillium wilt extract. Total DNA from the ten hybrids was restricted and hybridized with a 5.9 kb Oenothera chloroplast cytochrome f gene probe, a 2.4 kb EcoRI clone en

Who reads Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum?

Author

A. Guri; K. C. Sink

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)

Published

1988

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)