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Grape seed proanthocyanidins protect cardiomyocytes from ischemia and reperfusion injury via Akt-NOS signaling by Zuo-Hui Shao; Kimberly R. Wojcik; Anar Dossumbekova; Chinwang Hsu; Sangeeta R. Mehendale; Chang-Qing Li; Yimin Qin; Willard W. Sharp; Wei-Tien Chang; Kimm J. Hamann; Chun-Su Yuan; Terry L. Vanden Hoek is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Grape seed proanthocyanidins protect cardiomyocytes from ischemia and reperfusion injury via Akt-NOS signaling about?
## Abstract Ischemia/reperfusion (I/R) injury in cardiomyocytes is related to excess reactive oxygen species (ROS) generation and can be modulated by nitric oxide (NO). We have previously shown that grape seed proanthocyanidin extract (GSPE), a naturally occurring antioxidant, decreased ROS and may potentially stimulate NO production. In this study, we investigated whether GSPE administration at reperfusion was associated with cardioprotection and enhanced NO production in a cardiomyocyte I/R model. GSPE attenuated I/R‐induced cell death [18.0 ± 1.8% (GSPE, 50 μg/ml) vs. 42.3 ± 3.0% (I/R control), __P__ < 0.001], restored contractility (6/6 vs. 0/6, respectively), and increased NO release. The NO synthase (NOS) inhibitor Nω‐nitro‐L‐arginine methyl ester (L‐NAME, 200 μM) significantly reduced GSPE‐induced NO release and its associated cardioprotection [32.7 ± 2.7% (GSPE + L‐NAME) vs. 18.0 ± 1.8% (GSPE alone), __P__ < 0.01]. To determine whether GSPE induced NO production was mediated by the Akt‐eNOS pathway, we utilized the Akt inhibitor API‐2. API‐2 (10 μM) abrogated GSPE‐induced protection [44.3% ± 2.2% (GSPE + API‐2) vs. 27.0% ± 4.3% (GSPE alone), __P__ < 0.01], attenuated the en
Who reads Grape seed proanthocyanidins protect cardiomyocytes from ischemia and reperfusion injury via Akt-NOS signaling?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Zuo-Hui Shao; Kimberly R. Wojcik; Anar Dossumbekova; Chinwang Hsu; Sangeeta R. Mehendale; Chang-Qing Li; Yimin Qin; Willard W. Sharp; Wei-Tien Chang; Kimm J. Hamann; Chun-Su Yuan; Terry L. Vanden Hoek
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)