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Structure of a Monoclinic Crystal Form of Cytochrome b1 (Bacterioferritin) from E. coli by Alain Dautant; Jean-Brice Meyer; Joseph Yariv; Gilles Précigoux; Robert M. Sweet; A. Joseph Kalb; Felix Frolow is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Structure of a Monoclinic Crystal Form of Cytochrome b1 (Bacterioferritin) from E. coli about?

Crystals of E. coli cytochrome b1, alias bacterioferritin, were grown from a low ionic strength solution. The resulting monoclinic P21 structure was solved by molecular replacement and refined using noncrystallographic symmetries applied to the fundamental unit, consisting of two protein subunits and a single haem. From the Patterson self-rotation results it was shown that the asymmetric unit of the monoclinic crystal consists of 12 such dimers and corresponds to a complete, nearly spherical, molecule of bacterioferritin (Mr = 450 kDa) of 432 point-group symmetry. It is thus the most symmetrical cytochrome. As previously determined for the tetragonal form, the haem is located in a special position on a local twofold axis of the dimer. A bimetal centre is also observed within the four-helix bundle of each monomer; a metal-binding site is located on the fourfold axis.

Who reads Structure of a Monoclinic Crystal Form of Cytochrome b1 (Bacterioferritin) from E. coli?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Alain Dautant; Jean-Brice Meyer; Joseph Yariv; Gilles Précigoux; Robert M. Sweet; A. Joseph Kalb; Felix Frolow
Publisher
International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
Published
1998
Language
EN
ISBN
9780907444992
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

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