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Biodegradable Poly(ester Amine) Based on Glycerol Dimethacrylate and Polyethylenimine as a Gene Carrier by Rohidas B. Arote; Soon‐Kyung Hwang; Mi‐Kyong Yoo; Dhananjay Jere; Hu‐Lin Jiang; You‐Kyoung Kim; Yun‐Jai Choi; Jae‐Woon Nah; Myung‐Haing Cho; Chong‐Su Cho is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biodegradable Poly(ester Amine) Based on Glycerol Dimethacrylate and Polyethylenimine as a Gene Carrier about?
## Abstract ## Background Polyethylenimine (PEI) vectors are widely used in gene delivery because of their high transfection efficiency owing to a unique proton sponge effect. An increase in molecular weight increases transfection efficiency, but simultaneously results in increased toxicity. Therefore, the design and synthesis of new degradable gene delivery carriers having high transfection efficiencies and reduced cytotoxicity are necessary. ## Methods In the present study degradable poly(ester amines) (PEAs) based on glycerol dimethacrylate (GDM) and low molecular weight branched polyethylenimine (LMW‐PEI) were synthesized in anhydrous methanol at 60 °C following Michael addition reaction. The transfection efficiencies of the synthesized PEA/DNA complexes were evaluated using three different cell lines (HeLa, HepG2 and 293T cells) __in vitro__. ## Results PEAs with zeta potential in the range of 30–55 mV (at physiological pH) condensed plasmid DNA into nanosized particles (<150 nm) suitable for intracellular delivery. The PEAs degraded in a controlled fashion (__t__~1/2~ of approximately 9–10 days). Compared with PEI 25K, the PEAs showed significantly lower cytotoxicity in three
Who reads Biodegradable Poly(ester Amine) Based on Glycerol Dimethacrylate and Polyethylenimine as a Gene Carrier?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rohidas B. Arote; Soon‐Kyung Hwang; Mi‐Kyong Yoo; Dhananjay Jere; Hu‐Lin Jiang; You‐Kyoung Kim; Yun‐Jai Choi; Jae‐Woon Nah; Myung‐Haing Cho; Chong‐Su Cho
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)