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Can I read IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production on EtoBox?
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production by Guilherme Inocêncio Matos; de J Fernandes Claudia Covas; de Cássia Rita Bittar; Adriano Gomes-Silva; Fabiana Marques; Viviane C Maniero; Valdir S Amato; Manoel P Oliveira-Neto; Marise da Silva Mattos; Claude Pirmez; Elizabeth P Sampaio; Milton O Moraes; Alda Maria Da-Cruz is a Medicine article available to read on EtoBox.
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## Background Interferon-gamma is a key cytokine in the protective responses against intracellular pathogens. A single nucleotide polymorphism (SNP) located in the first intron of the human IFN-γ gene can putatively influence the secretion of cytokine with an impact on infection outcome as demonstrated for tuberculosis and other complex diseases. Our aim was to investigate the putative association of IFNG+874T/A SNP with American tegumentary leishmaniasis (ATL) and also the influence of this SNP in the secretion of IFN-γ __in vitro__. ## Methods Brazilian ATL patients (78 cutaneous, CL, and 58 mucosal leishmaniasis, ML) and 609 healthy volunteers were evaluated. The genotype of +874 region in the IFN-γ gene was carried out by Amplification Refractory Mutational System (ARMS-PCR). __Leishmania__-induced IFN-γ production on peripheral blood mononuclear cell (PBMC) culture supernatants was assessed by ELISA. ## Results There are no differences between +874T/A SNP frequency in cases and controls or in ML versus CL patients. Cutaneous leishmaniasis cases exhibiting AA genotype produced lower levels of IFN-γ than TA/TT genotypes. In mucosal cases, high and low IFN-γ producers were clearl
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- Author
- Guilherme Inocêncio Matos; de J Fernandes Claudia Covas; de Cássia Rita Bittar; Adriano Gomes-Silva; Fabiana Marques; Viviane C Maniero; Valdir S Amato; Manoel P Oliveira-Neto; Marise da Silva Mattos; Claude Pirmez; Elizabeth P Sampaio; Milton O Moraes; Alda Maria Da-Cruz
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2334)
- Published
- 2007
- Language
- EN
- Field
- Medicine (Health Sciences)