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Systematic engineering of phytochelatin synthesis and arsenic transport for enhanced arsenic accumulation in E. coli by Shailendra Singh; Seung Hyun Kang; Wonkyu Lee; Ashok Mulchandani; Wilfred Chen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Phytochelatin (PC) is a naturally occurring peptide with high affinity towards arsenic (As). In this article, we demonstrated the systematic engineering of PC‐producing __E. coli__ for As accumulation by addressing different bottlenecks in PC synthesis as well as As transport. Phytochelatin synthase from __Schizosaccharomyces pombe__ (SpPCS) was expressed in __E. coli__ resulting in 18 times higher As accumulation. PC production was further increased by co‐expressing a feedback desensitized γ‐glutamylcysteine synthetase (GshI\*), resulting in 30‐fold higher PC levels and additional 2‐fold higher As accumulation. The significantly increased PC levels were exploited further by co‐expressing an arsenic transporter GlpF, leading to an additional 1.5‐fold higher As accumulation. These engineering steps were finally combined in an arsenic efflux deletion __E. coli__ strain to achieve an arsenic accumulation level of 16.8 μmol/g DCW, a 80‐fold improvement when compared to a control strain not producing phytochelatins. Biotechnol. Bioeng. 2010. 105: 780–785. © 2009 Wiley Periodicals, Inc.

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Author

Shailendra Singh; Seung Hyun Kang; Wonkyu Lee; Ashok Mulchandani; Wilfred Chen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)