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Ganoderma Atrum Polysaccharide Protects Cardiomyocytes Against Anoxia/reoxygenation‐induced Oxidative Stress by Mitochondrial Pathway by Wen‐Juan Li; Shao‐Ping Nie; Yi Chen; Ming‐Yong Xie; Ming He; Qiang Yu; Yan Yan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract It is now well established that oxidative stress plays a causative role in the pathogenesis of anoxia/reoxygenation (A/R) injury. __Ganoderma atrum__ polysaccharide (PSG‐1), the most abundant component isolated from __G. atrum__, has been shown to possess potent antioxidant activity. The goals of this study were to investigate the effect of PSG‐1 against oxidative stress induced by A/R injury and the possible mechanisms in cardiomyocytes. In this work, primary cultures of neonatal rat cardiomyocytes pretreated with PSG‐1 were subjected to A/R and subsequently monitored for cell viability by the 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay. The levels of intracellular reactive oxygen species (ROS), apoptosis, and mitochondrial membrane potential (Δψ~m~) were determined by flow cytometry. Western blot analysis was used to measure the expression of cytochrome __c__, Bcl‐2 family, and manganese superoxide dismutase (MnSOD) proteins, and the activities of caspase‐3 and caspase‐9 were determined by a colorimetric method. The results showed that PSG‐1 protected against cell death caused by A/R injury in cardiomyocytes. PSG‐1 reduced the A/R‐indu

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Author

Wen‐Juan Li; Shao‐Ping Nie; Yi Chen; Ming‐Yong Xie; Ming He; Qiang Yu; Yan Yan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)