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Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium by Cynthia K Damer; Marina Bayeva; Emily S Hahn; Javier Rivera; Catherine I Socec is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium about?

## Background Copines are soluble, calcium-dependent membrane binding proteins found in a variety of organisms. Copines are characterized as having two C2 domains at the N-terminal region followed by an "A domain" at the C-terminal region. The "A domain" is similar in sequence to the von Willebrand A (VWA) domain found in integrins. The presence of C2 domains suggests that copines may be involved in cell signaling and/or membrane trafficking pathways. ## Results We have identified six copines genes in the __Dictyostelium discoideum__ genome, __cpnA-cpnF__, and have focused our studies on __cpnA__. CpnA is expressed throughout development and was shown to be capable of binding to membranes in a calcium-dependent manner __in vitro__. A GFP-tagged CpnA was also capable of binding to membranes in a calcium-dependent manner __in vitro__. In live wildtype __Dictyostelium__ cells expressing GFP-CpnA, GFP-CpnA was typically found in the cytoplasm without any specific localization to membranes. However, in a small subset of starved cells, GFP-CpnA was observed to bind transiently (typically ~1–10 s) to the plasma membrane and intracellular vacuoles. In some cells, the transient membrane loc

Who reads Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium?

Author

Cynthia K Damer; Marina Bayeva; Emily S Hahn; Javier Rivera; Catherine I Socec

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)