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Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice by Audrey C. Lam; Karen Li; Xiao B. Zhang; Chi K. Li; Tai F. Fok; Allan M.Z. Chang; Anthony E. James; Kam S. Tsang; Patrick M.P. Yuen is a Medicine article available to read on EtoBox.

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**BACKGROUND:** Ex vivo expansion of cord blood (CB) hematopoietic stem and progenitor cells increases cell dose and may reduce the severity and duration of neutropenia and thrombocytopenia after transplantation. This study's purpose was to establish a clinically applicable culture system by investigating the use of cytokines, serum‐free media, and autologous plasma for the expansion of CB cells and the engraftment of expanded product in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. **STUDY DESIGN AND METHODS:** Enriched CB CD34+ cells were cultured in four media (Iscove's modified Dul‐becco's medium with FCS, Gibco; X‐Vivo‐10, BioWhittaker; QBSF‐60, Quality Biological; and StemSpan SFEM, Stem Cell Technologies) with four cytokine combinations (thrombopoietin [TPO], SCF, Flt‐3 ligand [FL] with and without G–CSF, and/or IL‐6). The effect of autologous CB plasma was also investigated. The read‐out measures were evaluated on Days 8 and 12. After expansion at the optimized condition, cultured cells were transplanted into sublethally irradiated NOD/SCID mice. The engraftment of human CD45+ cells and subsets in the bone marrow, spleen, and peripheral blood was determ

Who reads Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice?

Author

Audrey C. Lam; Karen Li; Xiao B. Zhang; Chi K. Li; Tai F. Fok; Allan M.Z. Chang; Anthony E. James; Kam S. Tsang; Patrick M.P. Yuen

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

2001

Language

EN

Field

Medicine (Health Sciences)