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Inactivation of 12 viruses by heating steps applied during manufacture of a hepatitis B vaccine by Dr. P. N. Lelie; H. W. Reesink; C. J. Lucas is a Medicine article available to read on EtoBox.

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The efficacy of two heating cycles (90 sec at 103 O C and 10 hr at 65 "C) used during manufacture of a plasma-derived hepatitis-B vaccine was validated for the inactivation of 12 virus families. A period of 15 min warming up to 65 O C had already completely inactivated representatives of nine virus families, ie, poxvirus (vaccinia), picornavirus (encephalomyocarditis virus), togavirus (sindbis virus), coronavirus (mouse hepatitis virus), orthomyxovirus (influenza virus), rhabdovirus (vesicular stomatitis virus), herpes virus (cytomegalovirus), lentivirus (human immunodeficiency virus), and retrovirus (murine leukemia virus). After prolonged heating at 65 O C or heating for 90 sec at 103 "C, parvovirus (canine parvovirus) and the phage phiX174 were also completely inactivated. Papovavirus represented by simian virus 40 (SV-40) was the most heat-resistant virus evaluated. The infectivity of SV-40 was reduced by lo4 Tissue Culture Infectious Doses (TCIDSo) per ml after 90 sec at 103 O C , but a marginal residual activity ( t l . 5 TCID,, per ml) was observed. Subsequent pasteurization for 10 h at 65 "C did not further reduce the infectivity of SV-40. This study shows that the two heat

Who reads Inactivation of 12 viruses by heating steps applied during manufacture of a hepatitis B vaccine?

Author

Dr. P. N. Lelie; H. W. Reesink; C. J. Lucas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

1987

Language

EN

Field

Medicine (Health Sciences)