Opening book details…
Can I read CARD9 Deficiency Increases Hippocampal Injury Following Acute Neurotropic Picornavirus Infection but Does Not Affect Pathogen Elimination on EtoBox?
CARD9 Deficiency Increases Hippocampal Injury Following Acute Neurotropic Picornavirus Infection but Does Not Affect Pathogen Elimination by Suvarin Pavasutthipaisit; Melanie Stoff; Tim Ebbecke; Malgorzata Ciurkiewicz; Sabine Mayer-Lambertz; Theresa Störk; Kevin D. Pavelko; Bernd Lepenies; Andreas Beineke is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is CARD9 Deficiency Increases Hippocampal Injury Following Acute Neurotropic Picornavirus Infection but Does Not Affect Pathogen Elimination about?
Neurotropic viruses target the brain and contribute to neurologic diseases. Caspase recruitment domain containing family member 9 (CARD9) controls protective immunity in a variety of infectious disorders. To investigate the effect of CARD9 in neurotropic virus infection, CARD9^−/−^ and corresponding C57BL/6 wild-type control mice were infected with Theiler’s murine encephalomyelitis virus (TMEV). Brain tissue was analyzed by histology, immunohistochemistry and molecular analyses, and spleens by flow cytometry. To determine the impact of CARD9 deficiency on T cell responses in vitro, antigen presentation assays were utilized. Genetic ablation of CARD9 enhanced early pro-inflammatory cytokine responses and accelerated infiltration of T and B cells in the brain, together with a transient increase in TMEV-infected cells in the hippocampus. CARD9^−/−^ mice showed an increased loss of neuronal nuclear protein^+^ mature neurons and doublecortin^+^ neuronal precursor cells and an increase in β-amyloid precursor protein^+^ damaged axons in the hippocampus. No effect of CARD9 deficiency was found on the initiation of CD8^+^ T cell responses by flow cytometry and co-culture experiments using
Who reads CARD9 Deficiency Increases Hippocampal Injury Following Acute Neurotropic Picornavirus Infection but Does Not Affect Pathogen Elimination?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Suvarin Pavasutthipaisit; Melanie Stoff; Tim Ebbecke; Malgorzata Ciurkiewicz; Sabine Mayer-Lambertz; Theresa Störk; Kevin D. Pavelko; Bernd Lepenies; Andreas Beineke
- Publisher
- MDPI AG
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)