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Alleles of the proximal promoter of BAT1, a putative anti-inflammatory gene adjacent to the TNF cluster, reduce transcription on a disease-associated MHC haplotype by Agnes M.-L. Wong; Richard J. N. Allcock; Karey Y. M. Cheong; Frank T. Christiansen; Patricia Price is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Alleles of the proximal promoter of BAT1, a putative anti-inflammatory gene adjacent to the TNF cluster, reduce transcription on a disease-associated MHC haplotype about?

## Abstract **Background:** BAT1 belongs to the DEAD‐box family of proteins, and is encoded in the central region of the MHC, a region containing genes affecting immunopathological disorders including Type 1 diabetes. We showed that BAT1 can reduce inflammatory cytokine production, supporting its candidacy as a disease gene. Here we examined the proximal promoter region of __BAT1__. **Results:** Ten single nucleotide polymorphisms were identified in ≈1.4 kb of sequence, defining at least seven alleles. Sections of the __BAT1__ promoter region were amplified from cells homozygous for the MHC haplotypes associated with susceptibility (__HLA‐A1__, __B8__, __DR3__; 8.1 haplotype) and resistance (__HLA‐A3__, __B7__, __DR15__; 7.1 haplotype) to diabetes and cloned into a promoter‐less luciferase‐encoding plasmid. Jurkat cells transiently transfected with fragments from the 8.1 haplotype exhibited a lower luciferase activity than those transfected with fragments from the 7.1 haplotype, indicating reduced transcription. The effect was clearest with the 520 bp immediately upstream of the transcriptional start site. Electrophoretic mobility shift assays using oligonucleotides spanning polymo

Who reads Alleles of the proximal promoter of BAT1, a putative anti-inflammatory gene adjacent to the TNF cluster, reduce transcription on a disease-associated MHC haplotype?

Author

Agnes M.-L. Wong; Richard J. N. Allcock; Karey Y. M. Cheong; Frank T. Christiansen; Patricia Price

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)