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Reverse Transcription Recombinase-aided Amplification Assay for Avian Influenza Virus by Suchun Wang; Qingye Zhuang; Nan Jiang; Fuyou Zhang; Qiong Chen; Ran Zhao; Yang Li; Xiaohui Yu; Jinping Li; Guangyu Hou; Liping Yuan; Fuliang Sun; Zihao Pan; Kaicheng Wang is a Medicine article available to read on EtoBox.

What is Reverse Transcription Recombinase-aided Amplification Assay for Avian Influenza Virus about?

Avian influenza virus (AIV) infection can lead to severe economic losses in the poultry industry and causes a serious risk for humans. A rapid and simple test for suspected viral infection cases is crucial. In this study, a reverse transcription recombinase-aided amplification assay (RT-RAA) for the rapid detection of all AIV subtypes was developed. The reaction temperature of the assays is at 39 °C and the detection process can be completed in less than 20 min. The specificity results of the assay showed that this method had no cross-reaction with other main respiratory viruses that affect birds, including Newcastle disease virus (NDV) and infectious bronchitis virus (IBV). The analytical sensitivity at a 95% confidence interval was 10 2 RNA copies per reaction. In comparison with a published assay for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), the κ value of the RT-RAA assay in 384 avian clinical samples was 0.942 (p < 0.001). The sensitivity and specificity of the RT-RAA assay for avian clinical sample detection was determined as 97.59% (95% CI 93.55-99.23%) and 96.79% (95% CI 93.22-98.59%), respectively. The RT-RAA assay for AIV in this st

Who reads Reverse Transcription Recombinase-aided Amplification Assay for Avian Influenza Virus?

Author

Suchun Wang; Qingye Zhuang; Nan Jiang; Fuyou Zhang; Qiong Chen; Ran Zhao; Yang Li; Xiaohui Yu; Jinping Li; Guangyu Hou; Liping Yuan; Fuliang Sun; Zihao Pan; Kaicheng Wang

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Medicine (Life Sciences)