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A novel role for Bsd2 in the resistance of yeast to adriamycin by Tsutomu Takahashi; Takemitsu Furuchi; Akira Naganuma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In a search for undiscovered mechanisms of resistance to adriamycin, we screened a genomic library derived from __Saccharomyces cerevisiae__ for genes related to adriamycin resistance. To our surprise, we found that overexpression of __BSD2__ rendered yeast cells resistant to adriamycin. Downregulation of the metal transporters Smf1 and Smf2 is the only activity of Bsd2 reported to date, and Bsd2 deficiency increases intracellular levels of Smf1 and Smf2. __SMF2__‐disrupted cells exhibited significantly greater resistance to adriamycin, whereas the resistance of __SMF1__‐disrupted cells was only slightly improved. The sensitivity of the __SMF1__‐ and __SMF2__‐disrupted yeast cell line overexpressing __BSD2__ was almost the same as that of the __BSD2__‐overexpressing parental yeast cell. Thus the overexpression of __BSD2__ and the disruption of __SMF1__ and __SMF2__ might be involved in the same mechanism that confers resistance to adriamycin. Although both __SMF1__‐ and __SMF2__‐disrupted cells were very sensitive to EGTA, overexpression of __BSD2__ had little or no effect on sensitivity to EGTA. However, a partial decrease in the intracellular level of FLAG‐Smf2 was ob

Who reads A novel role for Bsd2 in the resistance of yeast to adriamycin?

Author

Tsutomu Takahashi; Takemitsu Furuchi; Akira Naganuma

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)