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Synthesis and Characterization of an Immobilized Phenylethanolamine N-Methyltransferase Liquid Chromatographic Stationary Phase by Nektaria Markoglou; Irving W. Wainer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Synthesis and Characterization of an Immobilized Phenylethanolamine N-Methyltransferase Liquid Chromatographic Stationary Phase about?

Norepinephrine is N-methylated to epinephrine by the catalytic effect of the terminal enzyme in catecholamine biosynthesis, phenylethanolamine N-methyltransferase (PNMT). PNMT has been covalently immobilized onto a silica-based liquid chromatographic support, glutaraldehyde-P (Glut-P). The resulting PNMT-Glut-P stationary phase (PNMT-SP) was enzymatically active, stable, and reusable. Standard Michaelis-Menten kinetic studies were performed with both free and immobilized PNMT and known substrates and inhibitors were examined. The results demonstrate that the PNMT-SP can be utilized for the rapid screening of potential PNMT substrates as well as the screening of compounds for PNMT inhibitory activity.

Who reads Synthesis and Characterization of an Immobilized Phenylethanolamine N-Methyltransferase Liquid Chromatographic Stationary Phase?

Author

Nektaria Markoglou; Irving W. Wainer

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)