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Can I read Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells on EtoBox?
Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells by Muhammad Dain Yazid; Shahrul Hisham Zainal Ariffin; Sahidan Senafi; Mohamad Abdul Razak; Rohaya Megat Abdul Wahab is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells about?
## Background: The main morphological features of primitive cells, such as stem and progenitor cells, are that these cells consists only one nucleus. The main purpose of this study was to determine the differentiation capacities of stem and progenitor cells. This study was performed using mononucleated cells originated from murine peripheral blood and MC3T3-E1 cells. Three approaches were used to determine their differentiation capacities: 1) Biochemical assays, 2) Gene expression analysis, and 3) Morphological observations. Results: We found that both cells were able to differentiate into mature osteoblasts, as assayed by ALP activity. RT-PCR analysis showed the activation of the Opn gene after osteoblast differentiation. Morphological observations of both cells revealed the formation of black or dark-brown nodules after von Kossa staining. Nevertheless, only mononucleated cells showed the significant increase in TRAP activity characteristic of mature osteoclasts. The osteoclast-specific CatK gene was only upregulated in mononucleated cells. Morphological observations indicated the existence of multinucleated osteoclasts. Sca-1 was activated only in undifferentiated mononucleated
Who reads Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Muhammad Dain Yazid; Shahrul Hisham Zainal Ariffin; Sahidan Senafi; Mohamad Abdul Razak; Rohaya Megat Abdul Wahab
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1475-2867)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)