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Enzyme Inhibitor Screening by Electrospray Mass Spectrometry with Immobilized Enzyme on Magnetic Silica Microspheres by Fengli Hu; Huiying Zhang; Huaqing Lin; Chunhui Deng; Xiangmin Zhang is a Chemistry article available to read on EtoBox.

What is Enzyme Inhibitor Screening by Electrospray Mass Spectrometry with Immobilized Enzyme on Magnetic Silica Microspheres about?

In this study, a novel technique for screening inhibitors by electrospray mass spectrometry (ESI-MS) with immobilized enzyme on magnetic microspheres has been demonstrated. First, the model enzyme acetylcholinesterase (AChE) is immobilized onto the 3-glycidoxypropyltrimethoxysilane (GLYMO)-modified magnetic silica microspheres. AChE activity was monitored by biochemical assay that is based on mixing of AChE immobilized microspheres and model substrate acetylcholine, separating and detecting the product through ESI-MS. Stability of the enzyme-immobilized microspheres was investigated. No apparent loss of enzyme activity was observed after fivefold reuse of AChE-immobilized microspheres. The enzymeimmobilized bioassay was used to effectively identify AChE inhibitors among two standard samples, huperzine A and huperzine B, and their source herbal Huperzia serrata, all of which were spiked into the substrate. The inhibition was determined by measuring a decrease of product formation using ESI-MS. (

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Author

Fengli Hu; Huiying Zhang; Huaqing Lin; Chunhui Deng; Xiangmin Zhang

Publisher

Elsevier Science; Springer-Verlag; Springer New York LLC; American Chemical Society (ACS); Springer Science and Business Media LLC; Elsevier BV (ISSN 1044-0305)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)