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Liquid Chromatographic–Electrospray Ionization–Mass Spectrometric Analysis of Cytochrome P450 Metabolites of Arachidonic Acid by Kasem Nithipatikom; Andrew J. Grall; Blythe B. Holmes; David R. Harder; John R. Falck; William B. Campbell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Liquid Chromatographic–Electrospray Ionization–Mass Spectrometric Analysis of Cytochrome P450 Metabolites of Arachidonic Acid about?

Arachidonic acid (AA) can be metabolized by cytochrome P450 (CYP) enzymes to many biologically active compounds including 5,6-, 8,9-, 11,12-, and 14,15epoxyeicosatrienoic acids (EETs), their corresponding dihydroxyeicosatrienoic acids (DHETs), and 20-hydroxyeicosatetraenoic acid (20-HETE). These eicosanoids are potent regulators of vascular tone. We developed a liquid chromatography-electrospray ionization-mass spectrometry method to simultaneously determine 5,6-, 8,9-, 11,12-, and 14,15-EETs; 5,6-, 8,9-, 11,12-, and 14,15-DHETs; and 20-HETE. [ 2 H 8 ]EETs, [ 2 H 8 ]DHETs, and [ 2 H 2 ]20-HETE were used as internal standards. These compounds are readily separated on a C 18 reverse-phase column using water:acetonitrile with 0.005% acetic acid as a mobile phase. The internal standards, [ 2 H 8 ]EETs, [ 2 H 8 ]DHETs, and [ 2 H 2 ]20-HETE, eluted slightly faster than the natural eicosanoids. The samples were ionized by electrospray with fragmentor voltage of 120 V and detected in a negative mode. The negative ion detection gave a lower background than the positive ion detection for these compounds. These eicosanoids exhibited high abundance of the ions corresponding to [M ؊ 1] ؊ . The

Who reads Liquid Chromatographic–Electrospray Ionization–Mass Spectrometric Analysis of Cytochrome P450 Metabolites of Arachidonic Acid?

Author

Kasem Nithipatikom; Andrew J. Grall; Blythe B. Holmes; David R. Harder; John R. Falck; William B. Campbell

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)