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Quantitative determination of pravastatin and its biotransformation products in human serum by turbo ion spray LC/MS/MS by Daniel Mulvana; Mohammed Jemal; Sabine Coates Pulver is a Chemistry article available to read on EtoBox.

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A sensitive, specific, accurate and reproducible analytical method was developed and validated to quantify pravastatin (Prav), pravastatin-d 5 (Prav-d 5 ), SQ-31906, SQ-31906-d 5 , and pravastatin lactone (Prav -Lac) in human serum samples. Serum samples (0.5 ml) were acidified and extracted by a solid-phase extraction procedure to isolate all five analytes from human serum. Sample extracts were reconstituted and analyzed by turbo ion spray liquid chromatography/tandem mass spectrometry (LC/MS/MS) in the positive ion mode. The total run time was 9 min between injections. The assay demonstrated a lower limit of quantitation (LLQ) of 0.5 ng/ml for all five analytes. The calibration curves were linear from 0.5 ng/ml to 100 ng/ml for all five analytes. The coefficients of determination of all calibration curves were ]0.999. Precision and accuracy quality control (QC) samples were prepared at concentrations of 2, 30, 80, and 500 ng/ml for all analytes. The intra-assay and inter-assay precision calculated from QC samples were within 8% for all analytes. The inter-assay accuracy calculated from QC samples was within 8% for all analytes. The extraction recoveries were ]90% for all analytes

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Author

Daniel Mulvana; Mohammed Jemal; Sabine Coates Pulver

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)