Can I read Suppression of proline-directed protein kinase FA systemically inhibits the growth of human acute leukemia cells on EtoBox?

Suppression of proline-directed protein kinase FA systemically inhibits the growth of human acute leukemia cells by Chih-Ping Hsu; Chuan-Ching Yang; Shiaw-Der Yang is a Medicine article available to read on EtoBox.

What is Suppression of proline-directed protein kinase FA systemically inhibits the growth of human acute leukemia cells about?

Initial studies revealed that proline-directed protein kinase F A (PDPK F A ) was overexpressed in various cancerous tissues relative to normal controls. However, the functional role of overexpressed PDPK F A in cancer remains to be established. In this report, we explore the potential role of PDPK F A in leukemia cell growth by investigating the effects of partial inhibition of this kinase on human acute promyelocytic leukemia (HL-60) and acute lymphoblastic leukemia (Jurkat) cells. Cloning of PDPK F A cDNA and its recombinant antisense expression vector and antibody were successfully developed. Several stable antisense clones of HL-60 and Jurkat cells were subcloned, which expressed a low level of PDPK F A when compared with the control-transfected clone in immunoblot analysis. Moreover, these antisense clones potently inhibited cell growth, clonogenic growth in soft agar and serum-independent growth. The results taken together demonstrate that suppression of PDPK F A is able to interfere with the growth of HL-60 and Jurkat cells, suggesting an essential role of this PDPK in human acute leukemia cell growth.

Who reads Suppression of proline-directed protein kinase FA systemically inhibits the growth of human acute leukemia cells?

Author

Chih-Ping Hsu; Chuan-Ching Yang; Shiaw-Der Yang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2001

Language

EN

Field

Medicine (Health Sciences)