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MicroRNA 452 regulates IL20RA-mediated JAK1/STAT3 pathway in inflammatory colitis and colorectal cancer by Santosh Lamichhane; Ji-Su Mo; Grinsun Sharma; Tae-Young Choi; Soo-Cheon Chae is a Medicine article available to read on EtoBox.

What is MicroRNA 452 regulates IL20RA-mediated JAK1/STAT3 pathway in inflammatory colitis and colorectal cancer about?

## Objective: Micrornas are a class of small, non-coding rnas that play a key role in several biological and molecular processes, including tumorigenesis. we previously identified that mir452 is upregulated in both colorectal cancer (crc) and colitis. however, the functional mechanisms of mir452 and its target genes in crc and colitis are not well understood. so, we hypothesize that mir452 can influence crc and dss-induced colitis model through the regulation of il20ra and its downstream jak-stats signaling pathway. ## Methods: We used a luciferase reporter assay to confirm the effect of mir452 on il20ra expression. the protein and mrna expression of a target gene and its associated molecules were measured by western blot, quantitative rt-pcr, and immunohistochemistry. ## Results: We found that the il20ra was a direct target gene of mir452. overexpression of mir452 in crc cell lines significantly decreased il20ra and its downstream janus kinase 1 (jak1), signal transducer and activator of transcription 1 (stat1) and stat3. knockdown of il20ra in crc cell lines by il20ra gene silencing also decreased the expression of il20ra, jak1, and stat3, but not of stat1. ## Conclusion: Our res

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Author

Santosh Lamichhane; Ji-Su Mo; Grinsun Sharma; Tae-Young Choi; Soo-Cheon Chae

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Medicine (Life Sciences)