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This document describes the scratch wound healing assay protocol to study cell migration during wound healing in vitro. The assay involves growing cells into a confluent monolayer, scratching the monolayer to mimic a wound, incubating to allow migration, and analyzing images over time to quantify the closure of the scratch width or wound area. The protocol involves cell culture preparation, creating scratches of consistent size, incubation for up to 24 hours to study migration without proliferation, stainin

Author

Neha Masarkar

Language

EN