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Critical Flux in Cross-flow Ultrafiltration of Protein Solutions by Sari Metsämuuronen; Marianne Nyström is a Environmental Science article available to read on EtoBox.

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The effect of solute size relative to membrane pore size on the critical flux during the ultrafiltration of protein solutions was investigated using the constant pressure method. Hydrophilic regenerated cellulose membranes with a cut-offof 10, 30 and 100 kg mor t, model proteins and skimmed milk solutions were used. The critical flux mainly increased with the pore size of the ultrafiltration membrane. The lowest critical fluxes, 40-50 L m -2 h-~, were obtained with the retentive 10 kg mol -t cut-off membrane. This membrane had a very low permeability and, thus, the critical fluxes were achieved at high transmembrane pressures (TMP): 1.7-2.3 bar. With the 100 kg mol-~ cut-offmembrane critical fluxes were obtained at 0.2 bar TMP, which were around 100 L m -2 h -), slightly declining with increasing protein molar mass. In skimmed milk experiments the permeate flux decreased when the protein molecules were cnzymaticaUy split to pcptides. A critical flux for skimmed milk solution could not be found unless the protein concentration was diluted to 0.3-w% or lower. The results with model proteins were then compared to those obtained with skimmed milk resulting in [3-1actoglobulin being the

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Author

Sari Metsämuuronen; Marianne Nyström

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0011-9164)

Published

2005

Language

EN

Field

Environmental Science (Physical Sciences)