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What is Sanger Dideoxy Chain Termination Method about?
The Sanger Dideoxy Method, developed by Fred Sanger in 1974, utilizes DNA polymerase and dideoxyribonucleotides to terminate DNA chain elongation during sequencing. The process involves preparing a reaction mixture with template DNA, primers, and nucleotides, which is then divided into four tubes containing different dideoxynucleotides to stop the synthesis at specific points. The resulting DNA fragments are analyzed through electrophoresis and detected via autoradiography.
- Author
- Upadesh Pattnaik
- Language
- EN