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Can I read Glycogen synthase kinase 3-β: A master regulator of toll-like receptor-mediated chronic intestinal inflammation on EtoBox?
Glycogen synthase kinase 3-β: A master regulator of toll-like receptor-mediated chronic intestinal inflammation by Claudia Hofmann; Nadja Dunger; Jürgen Schölmerich; Werner Falk; Florian Obermeier is a Medicine article available to read on EtoBox.
What is Glycogen synthase kinase 3-β: A master regulator of toll-like receptor-mediated chronic intestinal inflammation about?
Background: A disturbed regulation of Toll-like receptor (TLR) signal transduction resulting in the exclusive activation of proinflammatory signaling pathways may be critical for the perpetuation of established chronic colitis. Glycogen synthase kinase 3-b (GSK3-b) was recently identified as an important regulator of TLR signaling mediating excessive inflammatory responses. The aim of this study was to assess the role of GSK3-b activity in chronic intestinal inflammation. Methods: Chronic colitis was induced by dextran sodium sulfate (DSS) treatment. Mice were treated intraperitoneally with phosphate-buffered saline (PBS), CpG-ODN, or GSK3-b inhibitors (SB216763, LiCl). Intestinal inflammation was evaluated by histologic analysis and cytokine secretion of mesenteric lymph node cells (MLC). Nuclear extracts of MLC and lamina propria mononuclear cells (LPMC) were analyzed for nuclear factor kappaB (NF-jB) and CREB activity. Murine and human intestinal immune cells were stimulated in vitro with CpG-ODN, lipopolysaccharide (LPS), or anti-CD3 with or without LiCl. Results: GSK3-b blockade significantly reduced chronic intestinal inflammation and even abolished the colitis-intensifying e
Who reads Glycogen synthase kinase 3-β: A master regulator of toll-like receptor-mediated chronic intestinal inflammation?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Claudia Hofmann; Nadja Dunger; Jürgen Schölmerich; Werner Falk; Florian Obermeier
- Publisher
- John Wiley and Sons; Oxford University Press; Oxford University Press (OUP) (ISSN 1078-0998)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)