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Gram Staining Mechanism and Procedure is a document available to read on EtoBox.
The document describes the Gram staining technique used to differentiate between Gram-positive and Gram-negative bacteria. Gram-positive bacteria appear dark purple because their thick peptidoglycan cell wall prevents the loss of crystal violet dye during decolorization with ethanol. Gram-negative bacteria appear pink as their thinner peptidoglycan layer allows the crystal violet dye to be washed away by ethanol, leaving only the safranin counterstain visible. Lysozyme is an enzyme that can further hydrolyz
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