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Can I read A specific DNA unwinding activity associated with SV40 large T antigen. on EtoBox?
A specific DNA unwinding activity associated with SV40 large T antigen. by D. Giacherio; L.P. Hager is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A specific DNA unwinding activity associated with SV40 large T antigen. about?
The incubation of highly purified large T antigen with relaxed, circular SV40 DNA in the presence of topoisomerase I (nicking closing enzyme) resulted in the introduction of negative superhelical turns in the DNA. ATP was not required for this reaction. A similar introduction of superhelical turns could also be obtained when a recombinant plasmid DNA (Y182), which contains sequences from both SV40 DNA and pBR322, was used. However, no effect was observed when relaxed pBR322 DNA, which does not contain SV40 DNA sequences, was incubated with T antigen in the presence of topoisomerase. These results are consistent with the hypothesis that large T antigen can recognize and unwind specific sequences on SV40 DNA.
Who reads A specific DNA unwinding activity associated with SV40 large T antigen.?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- D. Giacherio; L.P. Hager
- Publisher
- Elsevier BV
- Published
- 1980
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)