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Interference with Cell Cycle Progression and Induction of Apoptosis by Dideoxynucleoside Analogs by Marina Viora; Gianfranco Di Genova; Roberto Rivabene; Walter Malorni; Andrea Fattorossi is a Immunology and Microbiology article available to read on EtoBox.

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The in vitro effect of single or combined doses of zidovudine (AZT) and dideoxycytidine (ddC) on PHA-activated human peripheral blood mononuclear cells (PBMC) proliferative response and lymphoblastoid T cell line CEM cell growth was evaluated. Clinically relevant amounts (0.1, 1 and 10/tM) of AZT, ddC and AZT/ddC combination (10 + 10 ~M) inhibited 3H TdR uptake in both cell models in a dose-dependent manner. The inhibitory effect on cell growth was confirmed by counting the amount of viable CEM cells recovered after 24, 48 and 72 h exposure to the drugs. On equimolar basis, ddC was considerably more efficient than AZT although the latter potentiates the activity of the former. Flow cytometric analysis of PBMC and CEM cells exposed to the dideoxynucleosides revealed a decrease in the rate of DNA synthesis (rate of passage through the S phase of the cell cycle) and a reduced number of cell generations, the latter assessed by measuring the halving of the fluorescent probe 5-6-carboxyfluorescein diacetate succinimidyl ester by flow cytometry. The analysis of CEM cells recovered after exposure to ddC or AZT/ddC combination (10+ 10 ~M), showed that in addition to perturbing cell cycle pr

Who reads Interference with Cell Cycle Progression and Induction of Apoptosis by Dideoxynucleoside Analogs?

Author

Marina Viora; Gianfranco Di Genova; Roberto Rivabene; Walter Malorni; Andrea Fattorossi

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0192-0561)

Published

1997

Language

EN

Field

Immunology and Microbiology (Life Sciences)