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Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban by Alcazar Magana, Armando; Wright, Kirsten; Vaswani, Ashish; Caruso, Maya; Reed, Ralph L.; Bailey, Conner F.; Nguyen, Thuan; Gray, Nora E.; Soumyanath, Amala; Quinn, Joseph; Stevens, Jan F.; Maier, Claudia S. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban about?
## Abstract ## Introduction The phytochemical composition of plant material governs the bioactivity and potential health benefits as well as the outcomes and reproducibility of laboratory studies and clinical trials. ## Objective The objective of this work was to develop an efficient method for the in‐depth characterisation of plant extracts and quantification of marker compounds that can be potentially used for subsequent product integrity studies. Centella asiatica (L.) Urb., an Ayurvedic herb with potential applications in enhancing mental health and cognitive function, was used as a case study. ## Methods A quadrupole time‐of‐flight analyser in conjunction with an optimised high‐performance liquid chromatography (HPLC) separation was used for in‐depth untargeted fingerprinting and post‐acquisition precursor ion quantification to determine levels of distinct phytochemicals in various C. asiatica extracts. ## Results We demonstrate the utility of this workflow for the characterisation of extracts of C. asiatica. This integrated workflow allowed the identification or tentative identification of 117 compounds, chemically interconnected based on Tanimoto chemical similarity, and the
Who reads Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Alcazar Magana, Armando; Wright, Kirsten; Vaswani, Ashish; Caruso, Maya; Reed, Ralph L.; Bailey, Conner F.; Nguyen, Thuan; Gray, Nora E.; Soumyanath, Amala; Quinn, Joseph; Stevens, Jan F.; Maier, Claudia S.
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)