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Osteopontin mediated eosinophils activation by group II innate lymphoid cells by Qingxiang Zeng; Luo Xi; Yinhui Zeng; Wenlong Liu; Lifeng Zhou is a Medicine article available to read on EtoBox.

What is Osteopontin mediated eosinophils activation by group II innate lymphoid cells about?

## Background Osteopontin (OPN) can regulate Th2 inflammation in allergic rhinitis (AR). A recent study suggested that group II innate lymphoid cells (ILC2s) were very important for airway inflammation. But the role of OPN in ILC2s regulation is not explored. ## Methods Purified ILC2s were stimulated by human recombinant OPN. The expression of GATA3 and RORα was assayed using real-time polymerase chain reaction (PCR) and enzyme linked immunosorbent assay. MiR-181a was transfected into eosinophils to test the OPN production. The protein concentrations of interleukin (IL)-5 and IL-13 were examined using ELISA. Purified eosinophils and ILC2s were cocultured and stimulated by OPN and the activation of eosinophils was detected by ELISA. ## Results After OPN stimulation, the ILC2s proliferation, the mRNA levels of GATA3 and RORα, the protein of GATA3, RORα, IL-5 and IL-13 expression were up-regulated significantly in a dose dependent manner. Eosinophils cultured alone transfected with miR-181a mimics produced less OPN protein compared with eosinophils transfected with miR-control, whereas OPN production was significantly promoted when miR-181a inhibitor was transfected. In the eosinophil

Who reads Osteopontin mediated eosinophils activation by group II innate lymphoid cells?

Author

Qingxiang Zeng; Luo Xi; Yinhui Zeng; Wenlong Liu; Lifeng Zhou

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Medicine (Health Sciences)