Can I read Dual transcriptional control of claudin‐11 via an overlapping GATA/NF‐Y motif: Positive regulation through the interaction of GATA, NF‐YA, and CREB and negative regulation through the interaction of Smad, HDAC1, and mSin3A on EtoBox?

Dual transcriptional control of claudin‐11 via an overlapping GATA/NF‐Y motif: Positive regulation through the interaction of GATA, NF‐YA, and CREB and negative regulation through the interaction of Smad, HDAC1, and mSin3A by Wing‐Yee Lui; Elissa W.P. Wong; Yuanfang Guan; Will M. Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Dual transcriptional control of claudin‐11 via an overlapping GATA/NF‐Y motif: Positive regulation through the interaction of GATA, NF‐YA, and CREB and negative regulation through the interaction of Smad, HDAC1, and mSin3A about?

## Abstract The expression of claudin‐11, a key integral tight junction protein, is tightly regulated to ensure that the integrity of the seminiferous epithelium could be maintained during the translocation of spermatocytes at the blood–testis barrier at stages VIII–IX. In this study, we elucidate how the overlapping GATA/NF‐Y motif within the core promoter of claudin‐11 gene is modulated by differential binding of various transcription factors, resulting in dual transcriptional control. Using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay, we confirmed that GATA, nuclear factor YA (NF‐YA), and cAMP response element‐binding protein (CREB) form a complex in vivo and bind to the GATA/NF‐Y region to promote claudin‐11 gene transcription. Such gene activations were significantly reduced in the presence of siRNA specific to these transcription factors. GATA and CREB transactivation could be further modulated by the presence of Smad3 and Smad4 proteins. Binding of Smad proteins at the GATA/NF‐Y motif could repress the GATA and CREB transactivation of claudin‐11 gene. Such repression which required the recruitment and physical interactions of hi

Who reads Dual transcriptional control of claudin‐11 via an overlapping GATA/NF‐Y motif: Positive regulation through the interaction of GATA, NF‐YA, and CREB and negative regulation through the interaction of Smad, HDAC1, and mSin3A?

Author

Wing‐Yee Lui; Elissa W.P. Wong; Yuanfang Guan; Will M. Lee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)