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Employing Calorimetric Methods to Determine the Mechanism of the Invertase Maximal Activity Delay by Chan, W. K. Dindi ;Hicks, Benjamin ;Kaliappan, Alagammai ;Garbett, Nichola C. ;Hansen, Lee D. ;Kenealey, Jason D. is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Employing Calorimetric Methods to Determine the Mechanism of the Invertase Maximal Activity Delay about?
Invertase catalyzes the hydrolysis of sucrose to glucose and fructose. Invertase from Saccharomyces cerevisiae has been used in studies of the enzyme catalytic mechanism for more than a century. Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) were used to determine the kinetics and physical characteristics of invertase, respectively. Two subspecies of invertase with unique melting points of ∼57 and ∼65 °C are identified using DSC. The rate of invertase-catalyzed sucrose hydrolysis was measured with ITC. ITC has the distinct advantage of directly measuring the rate of reaction, which makes it easy to determine when the enzyme exhibits maximal activity. ITC revealed a previously unknown significant delay after the injections of sucrose before invertase reaches maximal activity. The delay in maximum activity was decreased by a statistically significant (p > 0.05) 85% by an increase in invertase concentration from 1 to 47 μg/mL. Additionally, increasing the temperature of the reaction mixture from 25 to 55 °C resulted in a 66% decrease in the delay to reach maximum activity. However, increasing the sucrose concentration had no effect of the delay in m
Who reads Employing Calorimetric Methods to Determine the Mechanism of the Invertase Maximal Activity Delay?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Chan, W. K. Dindi ;Hicks, Benjamin ;Kaliappan, Alagammai ;Garbett, Nichola C. ;Hansen, Lee D. ;Kenealey, Jason D.
- Publisher
- American Chemical Society (ACS)
- Published
- 2020
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)