Can I read STAT5B restrains human B-cell differentiation to maintain humoral immune homeostasis on EtoBox?

STAT5B restrains human B-cell differentiation to maintain humoral immune homeostasis by Simon J. Pelham; Maria Soledad Caldirola; Danielle T. Avery; Joseph Mackie; Geetha Rao; Florian Gothe; Timothy J. Peters; Antoine Guerin; David Neumann; Doris Vokurkova; Vivian Hwa; Wenming Zhang; Shu-Chen Lyu; Iris Chang; Monali Manohar; Kari C. Nadeau; Maria Isabel Gaillard; Liliana Bezrodnik; Violeta Iotova; Norberto Walter Zwirner; Mavel Gutierrez; Waleed Al-Herz; Christopher C. Goodnow; Alexander Vargas-Hernández; Lisa R. Forbes Satter; Sophie Hambleton; Elissa K. Deenick; Cindy S. Ma; Stuart G. Tangye is a Medicine article available to read on EtoBox.

What is STAT5B restrains human B-cell differentiation to maintain humoral immune homeostasis about?

## Background: Lymphocyte differentiation is regulated by coordinated actions of cytokines and signaling pathways. il-21 activates stat1, stat3, and stat5 and is fundamental for the differentiation of human b cells into memory cells and antibody-secreting cells. while stat1 is largely nonessential and stat3 is critical for this process, the role of stat5 is unknown. ## Objectives: This study sought to delineate unique roles of stat5 in activation and differentiation of human naive and memory b cells. ## Methods: Stat activation was assessed by phospho-flow cytometry cell sorting. differential gene expression was determined by rna-sequencing and quantitative pcr. the requirement for stat5b in b-cell and cd4 t-cell differentiation was assessed using crispr-mediated stat5b deletion from b-cell lines and investigating primary lymphocytes from individuals with germline stat5b mutations. ## Results: Il-21 activated stat5 and strongly induced socs3 in human naive, but not memory, b cells. deletion of stat5b in b-cell lines diminished il-21-mediated socs3 induction. pbmcs from stat5b-null individuals contained expanded populations of immunoglobulin class-switched b cells, cd21tbet b cells,

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Author

Simon J. Pelham; Maria Soledad Caldirola; Danielle T. Avery; Joseph Mackie; Geetha Rao; Florian Gothe; Timothy J. Peters; Antoine Guerin; David Neumann; Doris Vokurkova; Vivian Hwa; Wenming Zhang; Shu-Chen Lyu; Iris Chang; Monali Manohar; Kari C. Nadeau; Maria Isabel Gaillard; Liliana Bezrodnik; Violeta Iotova; Norberto Walter Zwirner; Mavel Gutierrez; Waleed Al-Herz; Christopher C. Goodnow; Alexander Vargas-Hernández; Lisa R. Forbes Satter; Sophie Hambleton; Elissa K. Deenick; Cindy S. Ma; Stuart G. Tangye

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Medicine (Health Sciences)