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Deletion of the chromatin remodeling gene SPT10 sensitizes yeast cells to a subclass of DNA-damaging agents by Kaouther Tounekti; Mustapha Aouida; Anick Leduc; Jeremie Poschmann; Xiaoming Yang; Omrane Belhadj; Dindial Ramotar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The __Saccharomyces cerevisiae__ SPT10 protein possesses a DNA‐binding domain that is fused to a putative histone acetyltransferase domain. It binds specifically to upstream‐activating sequence elements in the core histone promoters and plays a direct role in histone gene regulation. SPT10 is also required for cell‐cycle‐specific K56 acetylation at histone genes, allowing the recruitment of the nucleosome remodeling factor Snf5 and subsequent regulation of gene transcription. We reisolated the __SPT10__ gene in a functional genome‐wide screen designed to identify haploid yeast mutants that are hypersensitive to the antitumor drug bleomycin, which acts by damaging DNA. In addition to bleomycin, we show that __spt10__Δ mutants are also hypersensitive to a limited set of genotoxic agents that create DNA strand breaks, but not to 254‐nm ultraviolet light or 4‐nitroquinoline‐1‐oxide, which generate helix distortion. The hypersensitivities of the __spt10__Δ mutant to the genotoxic agents are rescued by a single copy plasmid carrying the __SPT10__ gene. We further showed that __spt10__Δ mutants displayed a modest twofold increase spontaneous mutant frequency, as compared to th

Who reads Deletion of the chromatin remodeling gene SPT10 sensitizes yeast cells to a subclass of DNA-damaging agents?

Author

Kaouther Tounekti; Mustapha Aouida; Anick Leduc; Jeremie Poschmann; Xiaoming Yang; Omrane Belhadj; Dindial Ramotar

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0893-6692)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)