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Can I read Development and Validation of Fluorescent Receptor Assays Based on the Human Recombinant Estrogen Receptor Subtypes Alpha and Beta on EtoBox?
Development and Validation of Fluorescent Receptor Assays Based on the Human Recombinant Estrogen Receptor Subtypes Alpha and Beta by Theo de Boer; Debby Otjens; Alex Muntendam; Eric Meulman; Martine van Oostijen; Kees Ensing is a Chemistry article available to read on EtoBox.
What is Development and Validation of Fluorescent Receptor Assays Based on the Human Recombinant Estrogen Receptor Subtypes Alpha and Beta about?
This article describes the development and validation of two fluorescent receptor assays for the hRec-estrogen receptor subtypes alpha and beta. As a labelled ligand an autofluorescent phyto-estrogen (coumestrol) has been used. The estrogen receptor (ER) belongs to the nuclear receptor family, a class of soluble DNA binding proteins, mainly present in the cytoplasm of the cell, that act as ligand-activated enhancer factors. It consists of two different forms, expressed as ER-␣ (66 kDa) and ER- (59 kDa). The ER-␣ is mainly located in the uterus and the ER- can be found in vascular tissue. Detection and identification of compounds having estrogenic effects is of importance in drug discovery programmes within the pharmaceutical industry for their search for ER-subtype selective (ant)agonists which may prove to be of therapeutic value in treating a variety of estrogen-linked pathologies (breast cancer, osteoporosis, cardiovascular disease, type II diabetes and Alzheimer disease). Furthermore, interactions of (xeno-)estrogens with the endogenous hormonal system of the exposed organism can affect embryos, gonads, and reproductive behaviour. The latter can eventually lead to reduced rep
Who reads Development and Validation of Fluorescent Receptor Assays Based on the Human Recombinant Estrogen Receptor Subtypes Alpha and Beta?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Theo de Boer; Debby Otjens; Alex Muntendam; Eric Meulman; Martine van Oostijen; Kees Ensing
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)