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Dual Regulation of a Heat Shock Promoter During Embryogenesis: Stage‐dependent Role of Heat Shock Elements by Concepción Almoguera; Pilar Prieto‐Dapena; Juan Jordano is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary Transgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of a __s__mall heat shock protein (sHSP) gene from sunflower: __Ha hsp17.7 G4__. The promoter was developmentally regulated during zygotic embryogenesis and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) binding. They abolished the heat shock response of __Ha hsp17.7 G4__ and produced expression changes that demonstrated dual regulation of this promoter during embryogenesis. Thus, whereas activation of the chimeric genes during early maturation stages did not require intact HSE, expression at later desiccation stages was reduced by mutations in both the proximal (–57 to –89) and distal (–99 to –121) HSE. In contrast, two point mutations in the proximal HSE that did not severely affect gene expression during zygotic embryogenesis, eliminated the heat shock response of the same chimeric gene in vegetative organs. Therefore, by site‐directed mutagenesis, it was possible to separate the heat shock response of __Ha hsp17.7 G4__ from its developmental regula

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Author

Concepción Almoguera; Pilar Prieto‐Dapena; Juan Jordano

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)