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## 284 ANTIQENIC FRACTIONS OF PROTEUS alkaline solution. described the isolation, by extraction with trichloroacetic acid, of a complex antigen, claimed to be responsible for the We&Felix reaction. A discussion of some of the points at which the results presented here are at variance with previous reports will be found later in the paper. EXPERIMENTAL ## Cultivation of Organimns Proteus OX-19 was obtained from the American Type Culture Collection (No. 6380). The best yield of organisms rich in antigenic material was obtained on nutrient agar composed of 2 per cent of Bacto-tryptone, 0.5 per cent of a yeast concentrate,' 0.5 per oent of sodium chloride, and 3 per cent of Bacto-agar in tap water. Roux bottles containing 150 cc. of this medium were sterilized by autoclaving (30 minutes at 15 pounds). An inoculum of 3 cc. of a 24 hour culture was added to each bottle and the cultures were incubated for 24 to 36 hours at 37'. Following the customary bacteriological controls with respect to homogeneity, non-motility, etc., the heavy cultures were removed with physiological saline containing 0.2 per cent of formaldehyde. The organisms were washed three times in the centrifuge with formoli

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Author

Aaron Bendich; Erwin Chargaff

Publisher

Elsevier BV

Published

1946

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)