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Newly-developed Sendai virus vector for retinal gene transfer: reduction of innate immune response via deletion of all envelope-related genes by Yusuke Murakami; Yasuhiro Ikeda; Yoshikazu Yonemitsu; Sakura Tanaka; Haruhiko Kondo; Shinji Okano; Ri-ichiro Kohno; Masanori Miyazaki; Makoto Inoue; Mamoru Hasegawa; Tatsuro Ishibashi; Katsuo Sueishi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Newly-developed Sendai virus vector for retinal gene transfer: reduction of innate immune response via deletion of all envelope-related genes about?

## Abstract ## Background Recombinant Sendai virus vectors (rSeV) constitute a new class of cytoplasmic RNA vectors that have shown efficient gene transfer in various organs, including retinal tissue; however, the related immune responses remain to be overcome in view of clinical applications. We recently developed a novel rSeV from which all envelope‐related genes were deleted (rSeV/dFdMdHN) and, in the present study, assess host immune responses following retinal gene transfer. ## Methods rSeV/dFdMdHN or conventional F‐gene deleted rSeV (rSeV/dF) was injected into subretinal space of adult Wistar rats or C57BL/6 mice. The transgene expression and histopathological findings were assessed at various time points. Immunological assessments, including the expression of proinflammatory cytokines, natural killer (NK)‐cell activity, as well as SeV‐specific cytotoxic T lymphocytes (CTLs) and antibodies, were performed following vector injection. ## Results rSeV/dFdMdHN showed high gene transfer efficiency into the retinal pigment epithelium at an equivalent level to that seen with rSeV/dF. In the early phase, the upregulation of proinflammatory cytokines, local inflammatory cell infiltrat

Who reads Newly-developed Sendai virus vector for retinal gene transfer: reduction of innate immune response via deletion of all envelope-related genes?

Author

Yusuke Murakami; Yasuhiro Ikeda; Yoshikazu Yonemitsu; Sakura Tanaka; Haruhiko Kondo; Shinji Okano; Ri-ichiro Kohno; Masanori Miyazaki; Makoto Inoue; Mamoru Hasegawa; Tatsuro Ishibashi; Katsuo Sueishi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)