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Can I read Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation on EtoBox?
Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation by Wei Yang; Li-Bo Liu; Feng-Liang Liu; Yan-Hua Wu; Zi-Da Zhen; Dong-Ying Fan; Zi-Yang Sheng; Zheng-Ran Song; Jia-Tong Chang; Yong-Tang Zheng; Jing An; Pei-Gang Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation about?
Zika virus (ZIKV) is a potential threat to male reproductive health but the mechanisms underlying its influence on testes during ZIKV infection remain obscure. To address this question, we perform single-cell RNA sequencing using testes from ZIKV-infected mice. The results reveal the fragility of spermatogenic cells, especially spermatogonia, to ZIKV infection and show that the genes of the complement system are significantly upregulated mainly in infiltrated S100A4 + monocytes/macrophages. Complement activation and its contribution to testicular damage are validated by ELISA, RT‒qPCR and IFA and further verify in ZIKV-infected northern pigtailed macaques by RNA genome sequencing and IFA, suggesting that this might be the common response to ZIKV infection in primates. On this basis, we test the complement inhibitor C1INH and S100A4 inhibitors sulindac and niclosamide for their effects on testis protection. C1INH alleviates the pathological change in the testis but deteriorates ZIKV infection in general. In contrast, niclosamide effectively reduces S100A4 + monocyte/macrophage infiltration, inhibits complement activation, alleviates testicular damage, and rescues the fertility of ma
Who reads Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Wei Yang; Li-Bo Liu; Feng-Liang Liu; Yan-Hua Wu; Zi-Da Zhen; Dong-Ying Fan; Zi-Yang Sheng; Zheng-Ran Song; Jia-Tong Chang; Yong-Tang Zheng; Jing An; Pei-Gang Wang
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)