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Can I read Myosin-1a Powers the Sliding of Apical Membrane Along Microvillar Actin Bundles on EtoBox?
Myosin-1a Powers the Sliding of Apical Membrane Along Microvillar Actin Bundles by Russell E. McConnell; Matthew J. Tyska is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Myosin-1a Powers the Sliding of Apical Membrane Along Microvillar Actin Bundles about?
Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of investigation, the function of this unique actomyosin array has remained unclear. Here, we show that addition of ATP to isolated BBs induces a plus end–directed translation of apical membrane along microvillar actin bundles. Upon reaching microvillar tips, membrane is “shed” into solution in the form of small vesicles. Because this movement demonstrates the polarity, velocity, and nucleotide dependence expected for a Myo1a-driven process, and BBs lacking Myo1a fail to undergo membrane translation, we conclude that Myo1a powers this novel form of motility. Thus, in addition to providing a means for amplifying apical surface area, we propose that microvilli function as actomyosin contractile arrays that power the release of BB membrane vesicles into the intestinal lumen.
Who reads Myosin-1a Powers the Sliding of Apical Membrane Along Microvillar Actin Bundles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Russell E. McConnell; Matthew J. Tyska
- Publisher
- Rockefeller University Press.; The Rockefeller University Press; Rockefeller University Press; ScienceOpen (ISSN 0021-9525)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)