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Hydroxyl radical-induced cross-linking of thymine and lysine: Identification of the primary structure and mechanism by Syota Morimoto; Hiroshi Hatta; Shin-ichi Fujita; Tomochika Matsuyama; Tôru Ueno; Sei-ichi Nishimoto is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Hydroxyl radical-induced cross-linking of thymine and lysine: Identification of the primary structure and mechanism about?
Hydroxyl radical-induced formation of a cross-link of thymine (Thy) and lysine (Lys) in the y-radiolysis of N20-saturated aqueous solution was studied. A Thy-Lys cross-link (I) of the formal structure that OH radical and 4-carbon-centered Lys radical added respectively to C(5) and C(6) positions of Thy was isolated by a preparative HPLC and identified by a FAB-HRMS. The primary cross-link I was dehydrated by treatment with HC1 at 120 °C to yield the secondary structure (II) possessing a C(5)-C(6) double bond in the Thy moiety: the latter structure II was reported previously (Dizdaroglu, M.; Gajewski, E. Cancer Res. 1989, 49, 3463-3467). A pulse radiolysis study with a redox titration method indicated that 4-carbon centered Lys radical intermediate was of neutral redox reactivity in contrast to reducing reactivity of 5hydroxy-5,6-dihydrothymin-6-yl radical intermediate. The cross-link I could be formed by a conventional radical recombination mechanism, but not by an ionic recombination mechanism involving a redox reaction between the radical intermediates.
Who reads Hydroxyl radical-induced cross-linking of thymine and lysine: Identification of the primary structure and mechanism?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Syota Morimoto; Hiroshi Hatta; Shin-ichi Fujita; Tomochika Matsuyama; Tôru Ueno; Sei-ichi Nishimoto
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0960-894X)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)