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Genipin-crosslinked chitosan/alginate/alumina nanocomposite gels for 3D bioprinting by Jessica Condi Mainardi; Kurosch Rezwan; Michael Maas is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genipin-crosslinked chitosan/alginate/alumina nanocomposite gels for 3D bioprinting about?

## Abstract Immobilizing microorganisms inside 3D printed semi-permeable substrates can be desirable for biotechnological processes since it simplifies product separation and purification, reducing costs, and processing time. To this end, we developed a strategy for synthesizing a feedstock suitable for 3D bioprinting of mechanically rigid and insoluble materials with embedded living bacteria. The processing route is based on a highly particle-filled alumina/chitosan nanocomposite gel which is reinforced by (a) electrostatic interactions with alginate and (b) covalent binding between the chitosan molecules with the mild gelation agent genipin. To analyze network formation and material properties, we characterized the rheological properties and printability of the feedstock gel. Stability measurements showed that the genipin-crosslinked chitosan/alginate/alumina gels did not dissolve in PBS, NaOH, or HCl after 60 days of incubation. Alginate-containing gels also showed less swelling in water than gels without alginate. Furthermore, E. coli bacteria were embedded in the nanocomposites and we analyzed the influence of the individual bioink components as well as of the printing process

Who reads Genipin-crosslinked chitosan/alginate/alumina nanocomposite gels for 3D bioprinting?

Author

Jessica Condi Mainardi; Kurosch Rezwan; Michael Maas

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Physical Sciences)