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cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats by Hasan B Alam; Svetlana Stegalkina; Peter Rhee; Elena Koustova is a Medicine article available to read on EtoBox.

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The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies. ## Methods: Male sprague dawley rats (n = 25; 5/group) were subjected to a three stage hemorrhage and resuscitated as follows: (1) sham hemorrhage; (2) sham resuscitation; (3) lactated ringer's solution (lr), 3:1 volume; (4) 7.5% hypertonic saline (hts) 9.7 ml/kg; (5) plasma, 1:1 volume. liver, spleen, lung and muscle were collected 3 h post resuscitation and cdna array analysis was performed on the total rna. ## Results: Expression of 1,176 genes was analyzed. following resuscitation, 82 of the genes studied (7%) displayed an altered expression of at least 2-fold compared to the sham hemorrhage group. depending on organ system under study and resuscitation conditions, expression of these 82 genes was down- or up-regulated, bringing the total number of expression alterations to 167. largest number of organ-specific changes in gene expression was noted in liver (63/167), followed by lung (57), muscle (25), and spleen (22). most of the resuscitation strategy specific changes were caused by plasma resuscitati

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Author

Hasan B Alam; Svetlana Stegalkina; Peter Rhee; Elena Koustova

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-9572)

Published

2002

Language

EN

Field

Medicine (Health Sciences)