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Transformation of Coniothyrium minitans , a parasite of Sclerotinia sclerotiorum , with Agrobacterium tumefaciens by Moxiao Li; Xiaoyan Gong; Jin Zheng; Daohong Jiang; Yanping Fu; Mingsheng Hou is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Transformation of Coniothyrium minitans , a parasite of Sclerotinia sclerotiorum , with Agrobacterium tumefaciens about?

Coniothyrium minitans is a potential biological control agent of the plant pathogenic fungus Sclerotinia sclerotiorum. In this research, T-DNA insertional transformation of strain ZS-1 of C. minitans mediated by Agrobacterium tumefaciens was obtained, with optimization of spore maturity for transformation. After confirmation by PCR, transformants were subjected to Southern blot analysis, and results showed that more than 82.7% of transformants had single T-DNA insertions, and 12.1% of transformants had two copies T-DNA insertions. The genomic DNA segments of transformants flanking the T-DNA could be amplified from both borders with TAIL-PCR. Four types of mutants were screened and identified from the T-DNA insertional library, which comprised sporulation deficient mutants, pathogenicity deficient mutants, pigment change mutants and antibiotic deficient mutant, and some of the mutants were described; the number and frequency of each type of mutant from the library were calculated, and the frequency of each type is 3.27 x 10(-3), 1.0 x 10(-4), 1.4 x 10(-4), 2.5 x 10(-4), respectively. The successful creation of the T-DNA insertional transformation library may help us to unravel the i

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Author

Moxiao Li; Xiaoyan Gong; Jin Zheng; Daohong Jiang; Yanping Fu; Mingsheng Hou

Publisher

John Wiley and Sons; Oxford University Press; Oxford University Press (OUP); Elsevier BV (ISSN 0378-1097)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)