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Analysis of Phosphatidylcholine and Sphingomyelin Molecular Species from Brain Extracts Using Capillary Liquid Chromatography Electrospray Ionization Mass Spectrometry by Giorgis Isaac; Dan Bylund; Jan-Eric Månsson; Karin E. Markides; Jonas Bergquist is a Neuroscience article available to read on EtoBox.

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One feature of complex lipids is that many subtypes of these molecules exist as a diverse mixture in a biological sample. Qualitative and quantitative analysis of these closely related molecules require sensitive and specific analytical methods to detect intact phospholipids (PL) and sphingomyelin (SM) species and to differentiate between them. Conventional analytical methods require laborious procedures including separation by column, argentation thin-layer chromatography or liquid chromatography (LC) after pre-or post-column derivatization. In the present work, a method based on reversed phase capillary LC coupled on-line to electrospray ionization mass spectrometry (LC/ESI/MS) has been developed to gather tools for lipidomic studies, i.e. the profiling of complex mixtures of lipids in small amounts of various cells and tissues. The LC/MS system used consisted of an LC pump in an isocratic elution, a reversed phase capillary column and a single quadrupole mass spectrometer operated in the positive ion mode. A successful separation of phosphatidylcholine (PC) and SM molecular species was obtained with a minimum detectable quantity (MDQ) in the low fmol range injected on column. Th

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Author

Giorgis Isaac; Dan Bylund; Jan-Eric Månsson; Karin E. Markides; Jonas Bergquist

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)