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Efficient procedure of preparation and properties of long uniform G4–DNA nanowires by Natalia Borovok; Tatiana Molotsky; Jamal Ghabboun; Danny Porath; Alexander Kotlyar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Here we describe a novel and efficient procedure for preparation of long uniform G4-DNA wires. The procedure includes (i) enzymatic synthesis of double-stranded DNA molecules consisting of long (up to 10,000 bases), continuous G strands and chains of complementary (dC) 20 -oligonucleotides, poly(dG)-n(dC) 20 ; (ii) size exclusion HPLC separation of the G strands from the (dC) 20 oligonucleotides in 0.1 M NaOH; and (iii) folding of the purified G strands into G4-DNA structures by lowering the pH to 7.0. We show by atomic force microscopy (AFM) that the preparation procedure yielded G4-DNA wires with a uniform morphology and a narrow length distribution. The correlation between the total amount of nucleotides in the G strands and the contour length of the G4-DNA molecules estimated by AFM suggests monomolecular folding of the G strands into quadruplex structures. The folding takes place either in the presence or in the absence of stabilizing ions (K + or Na + ). The addition of these cations leads to a dramatic change in the circular dichroism spectrum of the G4-DNA.

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Author

Natalia Borovok; Tatiana Molotsky; Jamal Ghabboun; Danny Porath; Alexander Kotlyar

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)