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Identification of circ-FAM169A sponges miR-583 involved in the regulation of intervertebral disc degeneration by Li, Yongjin; Pan, Dayu; Liu, Shen; Xing, Xuewu; Zhou, Hengxing; Zhang, Bin; Zhang, Di; Li, Bo; Li, Guowang; Tao, Bo; Ning, Guangzhi; Feng, Shiqing is a Medicine article available to read on EtoBox.

What is Identification of circ-FAM169A sponges miR-583 involved in the regulation of intervertebral disc degeneration about?

## Summary ## Objective Low back pain (LBP) is the predominant cause of disc degeneration in patients, which brings serious social problems and economic burdens. Increasing evidence has indicated that intervertebral disc degeneration (IDD) is one of the most common causes triggering LBP. Accumulating evidence has shown that circRNAs are involved in the pathological process of IDD. Nevertheless, the circRNA-mediated IDD pathogenesis still remains unknown. This study explored the potential mechanism and functions of circ-FAM169A in NPCs. ## Methods Bioinformatics analysis was conducted to identify key circRNA, miRNA and mRNA and predict their potential role in IDD. Dual-luciferase reporter assay, western blot, qRT-PCR, and fluorescence __in situ__ hybridisation (FISH) were used to demonstrate the interaction among circ-FAM169A, miR-583 and Sox9 in NPCs. ## Results Herein, we identified circ-FAM169A, which was dramatically up-regulated in degenerative nucleus pulposus (NP) tissues and negatively correlated with expression levels of miR-583. We constructed a circ-FAM169A-miR-583-mRNAs co-expression network and predicted circ-FAM169A-miR-583 pathway predominantly involved in extracellul

Who reads Identification of circ-FAM169A sponges miR-583 involved in the regulation of intervertebral disc degeneration?

Author

Li, Yongjin; Pan, Dayu; Liu, Shen; Xing, Xuewu; Zhou, Hengxing; Zhang, Bin; Zhang, Di; Li, Bo; Li, Guowang; Tao, Bo; Ning, Guangzhi; Feng, Shiqing

Publisher

Elsevier ; Elsevier (Singapore) Pte Ltd; Elsevier BV (ISSN 2214-031X)

Published

2021

Language

EN

Field

Medicine (Health Sciences)